Composite

Part:BBa_K5366029:Design

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)


T7 promoter-RBS-MBP-linker-AJC7-6xHis -T7 termonator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 399
    Illegal BglII site found at 1677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2179
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 97


Design Notes

The DNA of AJC7 and MBP were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of NusA at the N-terminus of AJC7.


Source

none

References